This study fabricates a micro proton exchange membrane fuel cell (PEMFC) using micro electro mechanical systems (MEMS) technology. The active area of the membrane is 2 cm × 2 cm (4 cm2 ). The study is divided into two categories: [(1) the parametric experimental investigation, and (2) the durability test. This work is an attempt to find out how several parameters, including reheat temperature, the material of the current collector plates, the open ratio, and different cathode gases affect micro PEFMC performance. According to the experimental results obtained, both the conducting area and the material of the current collector plates exert great influences on the performance of the micro PEMFC, especially in the conducting area. The cell’s performance is finite when the gas reheat temperature is increased. The results show that the cell performance is better for an open ratio of 75% as compared to ratios of 50% and 67%. The concentration polarization is improved by increasing the air flow rate at high current densities, and if the GDL diffusive capability in the latter cell could be promoted, the differences between these two cells’ performances would be reduced. Furthermore, the performance at an operating voltage of 0.6 V was the most stable one among the four cases tested, and the performance deviation at a fixed operating voltage of 0.4 V was less than ±2.2%.